Direct determination of urinary mycotoxin biomarkers

Researchers have developed and applied salting-out assisted liquid and liquid extraction for multi-mycotoxin biomarkers analysis in pig urine. High performance liquid chromatography and tandem mass spectrometry were used in the analysis.

In this study, which results are published in the May 2013 edition of Journal of Chromatography A, a new analytical method was developed and validated for simultaneous analysis of aflatoxin B1, deoxynivalenol, fumonisin B1, ochratoxin A, zearalenone and T2 toxin and their metabolites in pig urine. In total 12 analytes were selected. A salting-out assisted liquid–liquid extraction procedure was used for sample preparation. High performance liquid chromatography and tandem mass spectrometry was used for the separation and detection of all the analytes.

The extraction recoveries were in a range of 70–108%, with the intra-day relative standard deviation and inter-day relative standard deviation lower than 25% for most of the compounds at 3 different concentration levels. Meanwhile the method bias for all the analytes did not exceed 20%. The limits of quantification ranged from 0.07 ng mL⁻¹ for ochratoxin A to 3.3 ng mL⁻¹ for deoxynivalenol. Matrix effect was evaluated in this study and matrix-matched calibration was used for quantification. The developed method was also validated for human urine as an extension of its application. Finally, the developed method was applied in a pilot study to analyse 28 pig urine samples. Deoxynivalenol, aflatoxin B1, fumonisin B1 and ochratoxin A were detected in these samples.

For more information see Science Direct